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Analytical Studies
Original
Papers |
Planta Med 2011;
77(1): 81-86
DOI: 10.1055/s-0030-1250132 |
© Georg Thieme Verlag KG
Stuttgart · New York |
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Quality
Assessment of Polygonum cuspidatum and
Polygonum multiflorum by 1H
NMR Metabolite Fingerprinting and Profiling
Analysis |
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| Michel Frédérich1,
Jean-Noël Wauters1,
Monique Tits1,
Charlotte Jason1,
Pascal de Tullio2,
Yvan Van der Heyden3,
Guorong Fan4,
Luc Angenot1 |
1 Drug Research
Center (CIRM), Laboratory of Pharmacognosy, University of
Liège, Liège, Belgium
2
Drug Research Center (CIRM), Laboratory of Medicinal
Chemistry, University of Liège, Liège, Belgium
3 Faculteit Geneeskunde en
Farmacie, Farmaceutisch Instituut, Dienst Analytische
Scheikunde en Farmaceutische Technologie (FABI), VUB,
Brussels, Belgium
4 School
of Pharmacy, Second Military Medical University, Shanghai, P.
R. China |
Abstract
The quality assessment and control of traditional Chinese
medicines (TCM) nowadays receives a great deal of attention
worldwide and particularly in Europe with its increasing local use.
Polygonum cuspidatum Siebold & Zucc. and
Polygonum multiflorum Thunb. are two members
of the Polygonaceae family, which are widely used as Chinese
medicinal plants. The aim of this study was to achieve an overview
of the quality of P. cuspidatum and P. multiflorum samples available on the Chinese
market and to identify important metabolites for their
discrimination, using 1H NMR-based metabolomics.
1H NMR and multivariate analysis techniques were applied
to almost 60 plant samples collected in different places in China.
Using 1H NMR metabolomics, it was possible, without
previous evaporation or separation steps, to obtain metabolic
fingerprints to distinguish between the species. The important
metabolites for discrimination were stilbene derivatives. Finally, a
clear distinction between the two species was possible and the
discriminant metabolites were identified.
Key words
metabolomics - 1H NMR - Polygonum
cuspidatum - Polygonum multiflorum -
Polygonaceae
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